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CRF1a and CRF2c are expressed in MCF7 cells as detected by RT-PCR. Total RNA was isolated from MCF7 cells and the expression of CRF receptors subtypes CRF1a, CRF1b, CRF2a and CRF2c were detected by semi-quantitative RT-PCR. The levels of beta Actin were also measured as a normalization control. Results are representative of three independent experiments.
