Fig. 5. GABA_B and mGluR5 receptors contribute to photothrombosis-induced astrocytic Ca²⁺ signaling.

A–C) Astrocytic Ca²⁺ signals following photothrombosis before (left) and after (right) administration of different antagonists. D) Summary of the effects of antagonists on photothrombosis-induced astrocytic Ca²⁺ signals. Ca²⁺ signals in the absence of pharmaceutical reagents were collected 30–60 minutes following photothrombosis. Ca²⁺ signals in the presence of pharmaceutical reagents were collected 20–60 minutes after administration of these drugs. In each group of experiment, we recorded Ca²⁺ signal up to 40 min following photothrombosis to establish the base line Ca²⁺ signal before adding drug. The data were averaged from N=4–6 mice in each condition. Statistical analysis by ANOVA with post-hoc tests indicated significant difference comparing effects with and without appropriate antagonists. *p<0.05.