Table 1.

Detection of shared TCRs within each of four matched pairs of mice adoptively transferred with single-donor splenocytes but not among unmatched pairs

TCRBV10 sequence			Repertoire in mouse pairs
BJ	NS	CDR3	No. of cells	% sort	% BJ	No. of cells	% sort	% BJ
			11A			11B
1S1	1.1-7a	SRGPEV	56	23.3	100	0	0	0
	1.1-3a	SLGQEV	0	0	0	59	30.7	100
1S2	1.2-11b	SWGSDY	24	10	50	0	0	0
	1.2-3a	SYGSDY	16	6.7	33.3	0	0	0
	1.2-9c	SQGSDY	8	3.3	16.7	4	2.1	20
	1.2-3c	SYGSDY	0	0	0	16	8.3	80
1S3	1.3-2a	SRGNTL	12	5	100	1	0.5	100
	Proportion amplified:		116/240	(48.3%)		80/192	(41.7%)
			12A			12B
1S1	1.1-4c	SFGTEV	0	0	0	1	0.5	100
1S2	1.2-2m	SLGSDY	44	22.9	77	51	26.0	100
	1.2-35a	SSGQDY	13	6.8	23	0	0	0
	Proportion amplified:		57/192	(29.7%)		52/192	(27.1%)
			13A			13B
1S2	1.2-73a	SFGASDY	64	33.3	74.4	5	2.6	100
	1.2-21	SLGSDY	22	11.5	25.6	0	0	0
	Proportion amplified:		86/192	(44.8%)		5/192	(2.6%)
			14A			14B
1S2	1.2-1h	SRGSDY	96	50	97	7	3.6	70
	1.2-76a	SFGTGENSDY	3	1.6	3	0	0	0
	1.2-2c	SLGSDY	0	0	0	3	1.6	30
1S3	1.3-1b	SLGNTL	3	1.6	100	1	0.5	100
1S4	1.4-11a	RPDREPPNERL	1	0.5	100	0	0	0
	1.4-12a	TGTGFSNERL	0	0	0	10	5.2	90.9
	1.4-9a	SLGQKL	0	0	0	1	0.5	9.1
	Proportion amplified:		103/192	(54.0%)		22/192	(11.5%)

Single VB10⁺CD62L ⁻CD8⁺ cells were sorted from PBL of four pairs of single-donor irradiated recipients on day 21 (12A/B and 13A/B) or 26 (pairs 11A/B and 14A/B) after injection of 500 P815-CW3 cells. The TCR VB10 junctional region sequences were determined after a modified single-cell PCR amplification protocol, described in Materials and Methods, which amplifies VB10 TCRs rearranged to either the JB1.1, −1.2, −1.3, or −1.4 gene elements. For each sequence, the number (no. cells) and the corresponding percentage (% sort) of positive PCR products of the total number of cells analyzed is shown, as is the percent of the sequence within the JB class (% BJ). The proportion of cells amplified is calculated for the total cells analyzed. Shared sequences are highlighted in bold type.