Table 2.
No shared TCRs detected among three mice adoptively transferred with multiple-donor splenocytes
| TCRBV10 sequence
|
Repertoire in multiple-donor recipients
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| BJ | NS | CDR3 | 21A
|
21B
|
21C
|
||||||
| No. of cells | % sort | % BJ | No. of cells | % sort | % BJ | No. of cells | % sort | % BJ | |||
| 1S1 | 1.1-6b | SLGQGV | 0 | 0 | 0 | 14 | 7.3 | 100 | 0 | 0 | 0 |
| 1.1-24a | RMGGLTTKV | 1 | 0.5 | 100 | 0 | 0 | 0 | 0 | 0 | 0 | |
| 1S2 | 1.2-1o | SRGSDY | 29 | 15.1 | 60.4 | 0 | 0 | 0 | 0 | 0 | 0 |
| 1.2-55a | SLGHLY | 9 | 4.7 | 18.8 | 0 | 0 | 0 | 0 | 0 | 0 | |
| 1.2-1n | SRGSDY | 6 | 3.1 | 12.5 | 0 | 0 | 0 | 0 | 0 | 0 | |
| 1.2-14c | SSGPDY | 3 | 1.6 | 6.3 | 0 | 0 | 0 | 0 | 0 | 0 | |
| 1.2-2i | SLGSDY | 1 | 0.5 | 2.1 | 0 | 0 | 0 | 0 | 0 | 0 | |
| 1.2-42e | SLGPDY | 0 | 0 | 0 | 45 | 23.4 | 51.7 | 0 | 0 | 0 | |
| 1.2-22b | STGTDY | 0 | 0 | 0 | 24 | 12.5 | 27.6 | 0 | 0 | 0 | |
| 1.2-58a | SAGTNY | 0 | 0 | 0 | 18 | 9.4 | 20.7 | 0 | 0 | 0 | |
| 1.2-64a | SLGQSY | 0 | 0 | 0 | 0 | 0 | 0 | 64 | 33.3 | 98.5 | |
| 1.2-1i | SRGSDY | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0.5 | 0.5 | |
| 1S3 | 1.3-3a | SFGNTL | 7 | 3.6 | 100 | 0 | 0 | 0 | 0 | 0 | 0 |
| 1S4 | 1.4-8a | SSGERL | 0 | 0 | 0 | 0 | 0 | 0 | 21 | 10.9 | 100 |
| Proportion amplified: | 55/192 | 101/192 | 86/192 | ||||||||
The TCR VB10 junctional region sequences expressed by single VB10+CD62L−CD8+ cells sorted from PBL of three multiple-donor irradiated recipients on day 20 after injection of 500 P815-CW3 cells were determined by the same protocol described for Table 1.