Table 4.
Distinct TCR repertoires selected in two control DBA/2 mice immunized with 500 P815-CW3 cells
| Control mouse | TCRBV10 sequence
|
Repertoire
|
||||
|---|---|---|---|---|---|---|
| BJ | NS | CDR3 | No. of cells | % sort | % BJ | |
| No. 32 | 1S1 | 1.1-12a | SFGTKV | 24 | 12.5 | 85.7 |
| 1.1-11b | SYGEEV | 2 | 1.0 | 7.1 | ||
| 1.1-3c | SLGQEV | 1 | 0.5 | 3.6 | ||
| 1.1-22a | SRGQEV | 1 | 0.5 | 3.6 | ||
| 1S2 | 1.2-6a | SFGSDY | 23 | 12.0 | 62.2 | |
| 1.2-66a | SVGDYY | 10 | 5.2 | 27.0 | ||
| 1.2-1p | SRGSDY | 1 | 0.5 | 2.7 | ||
| 1.2-2o | SLGSDY | 1 | 0.5 | 2.7 | ||
| 1.2-65a | SAGSDY | 1 | 0.5 | 2.7 | ||
| 1.2-74a | SLGHYY | 1 | 0.5 | 2.7 | ||
| 1S4 | 1.4-1a | SFGERL | 24 | 12.5 | 92.3 | |
| 1.4-7a | SSGTGL | 0.5 | 3.8 | |||
| 1.4-3c | SYGERL | 1 | 0.5 | 3.8 | ||
| Proportion amplified: | 91/192 | (47.4%) | ||||
| No. 8 | 1S1 | 1.1-3a | SLGQEV | 22 | 11.5 | 55.0 |
| 1.1-2a | SLGTEV | 14 | 7.3 | 35.0 | ||
| 1.1-4a | SFGTEV | 2 | 1.0 | 5.0 | ||
| 1.1-2f | SLGTEV | 1 | 0.5 | 2.5 | ||
| 1.1-23a | SLGERV | 1 | 0.5 | 2.5 | ||
| 1S2 | 1.2-42b | SLGPDY | 10 | 5.2 | 35.7 | |
| 1.2-3a | SYGSDY | 8 | 4.2 | 28.6 | ||
| 1.2-42f | SLGPDY | 4 | 2.1 | 14.3 | ||
| 1.2-1m | SRGSDY | 2 | 1.0 | 7.1 | ||
| 1.2-14c | SSGPDY | 2 | 1.0 | 7.1 | ||
| 1.2-2n | SLGSDY | 1 | 0.5 | 3.6 | ||
| 1.2-39b | SYGPDY | 1 | 0.5 | 3.6 | ||
| 1S3 | 1.3-1a | SLGNTL | 8 | 4.2 | 100 | |
| 1S4 | 1.4-4a | SLGERL | 6 | 3.1 | 100 | |
| Proportion amplified: | 82/192 | (42.7%) | ||||
The TCR VB10 junctional region sequences expressed by single VB10+CD62L−CD8+ cells sorted from PBL of DBA/2 mice on day 20 (no. 32) or day 22 (no. 8) after injection of 500 P815-CW3 cells were determined by the same protocol described for Table 1.