Table 1.
Effect of CaMKII on native and recombinant GABAA receptors
| Cells | Receptors | CaMKII prep | Phosphatase inhibitors | Kinase inhibitors | Plasticity | Other effects | Ref |
|---|---|---|---|---|---|---|---|
| Cerebellar Purkinje neurons | Native | Native, depol. stim. | Calyculin-A (PP1/PP2A) | Staurosporine, KN-62, calmodulin binding domain peptide (CBD), calmidazolium, heat inactivation | sIPSCs, IGABA | Amplitude enhanced | Kano et al. 1996 |
| Preactivated CaMKI | IGABA | Amplitude enhanced | |||||
| Dorsal horn spinal neurones | Native | Preactivated CaMKII | Calyculin-A (PP1, PP2A) | Heat inactivation | IGABA | Amplitude increased, Desensitisation reduced | Wang et al. 1995 |
| Hippocampal CA1 | eIPSPs | Enhanced amplitude | |||||
| HEK cells | Recombinant α1β2, α1β2γ2 | Preactivated CaMKII | — | — | IGABA | No modulation | Houston and Smart 2006 |
| α1β3, α1β3γ2 | IGABA | No modulation | |||||
| NG108-15 cells | Recombinant α1β2, α1β2γ2 | Preactivated CaMKII | — | — | IGABA | No modulation | Houston and Smart 2006 |
| α1β3, α1β3γ2 | IGABA | Enhanced | |||||
| Cerebellar granule neurons | Recombinant α1β2, α1β2γ2 | Preactivated CaMKII | — | — | IGABA | No modulation | Houston and Smart 2006 |
| α1β3, α1β3γ2 | IGABA | Enhanced | |||||
| NG108-15 cells | Recombinant α1β3S383A | Preactivated CaMKII | — | — | IGABA | No modulation | Houston et al. 2007 |
| α1β3S383Aγ2 | IGABA | Reduced modulation | |||||
| α1β3S383A+γ2Y365F, Y367F | IGABA | No modulation | |||||
| Cerebellar granule neurons | Native | Preactivated CaMKII | — | — | IGABA | Enhanced | Houston and Smart 2006 |
| Cerebellar granule neurons | Native β2−/− | Preactivated CaMKII | — | — | IGABA | Enhanced | Houston and Smart 2006 |
| Cerebellar granule neurons | Native | Preactivated CaMKII | — | — | sIPSCs | Amplitude enhanced, Duration prolonged | Houston et al. 2008 |
| Cerebellar granule neurons | Native β2−/− | Preactivated CaMKII | — | — | sIPSCs | Only duration prolonged | Houston et al. 2008 |
| Hippocampal CA1 | Native | Native +Ca2+/calmodulin | CaMKII autoinhib. pep. (281–301), BAPTA, Vincristine, Cytochalasin D, Colchicine | eIPSCs sIPSCs | Amplitude enhanced, Amplitude & Frequency enhanced | Wei et al. 2004 |
Summary of the effects of CaMKII on native neuronal GABAA receptors (wild-type and a β2 knock-out), and on recombinant GABAA receptors. The CaMKII preparations used are either: native, which is then activated by depolarising stimulation (depol. stim.) together in one study, with the intracellular addition of Ca2+ and calmodulin, or it is a preactivated form of CaMKII that is perfused internally into the cells. The effect of CaMKII was studied on whole-cell GABA-activated currents (IGABA) or spontaneous (s) or evoked (e) IPSCs, with one study using IPSPs.