Table 1.

Bestrophin and control primer sequences used in the RT-PCR amplification reactions

Gene	Sense (5′–3′)	Antisense (5′–3′)	Expected product size (bp)	GenBank accession
hBest1 5′	304-GTATTGCGACAGCTACATCCAG-327	794-ATCCAGTCGTAGGCATACAGGT-771	488	NM 004183
hBest1 mid	771-ACCTGTATGCCTACGACTGGAT-794	1233-CTGGAACTCCATCTCCTCTTTG-1210	460	NM 004183
hBest1 3′	1105-GGACATGTACTGGAATAAGCCC-1128	1514-GTGTCTGGGGCACTGTAGTC-1493	407	NM 004183
hBest2	443-AGTTTGAAAACCTGAACTCATCCTA-469	865-AGAAAGTTGGTCTCAAAGTCATCAT-839	420	NM 017682
hBest3	833-ACAAGTGACAGCTCCATGTTCTTAC-859	1350-TTTTAGAAAGGTATCACCAGGGTCT-1324	515	NM 032735
hBest4	957-TTTGAGACAAATCAGCTCATAGACC-983	1568-TCTTCTCTTTCAAGTTCTGTCCCTA-1544	609	NM 153274
β-Actin	260-GCATCCTCACCCTGAAGTAC-281	717-TTCTCCTTAATGTCACGCAC-697	455	NM 001101
GAPDH	627-ACCACAGTCCATGCCATCAC-648	1080-TCCACCACCCTGTTGCTGTA-1059	451	NM 002046

PCR primers for hBest1, 2, 3, 4, β-actin and GAPDH that have been used in this study. Primers were designed using the OligoPerfect™ primer design programme by Invitrogen.