Table 2.

Antibodies used in Western blotting and confocal microscopy analysis

Antibody	Supplier	Animal raised in	Target/Function	Peptide location	Sequence antibody raised against
Bst112	Fg	RP	hBest1	aa 445–464	WKLKAVDAFKSAPLYQRPGY
Bst121	Fg	RP	hBest1	aa 566–585	TLKDHMDPYWALENRDEAHS
BstMM	Ab	MM	hBest1	aa 568–585	KDHMDPYWALENRDEAHS
β-Actin	Ab	RP	β-actin	—	Target sequence between aa 1–100
Pan cadherin	Ab	MM	Pan cadherin/plasma membrane marker	aa 883–902	DYDYLNDWGPRFKKLADMYGGGDD
Calnexin	Ab	MM	Calnexin ER marker	—	Target sequence not divulged by supplier
GM130	Ab	MM	Golgi marker	—	Target sequence not divulged by supplier
Clathrin	Ab	MM	Vesicular marker	—	Target sequence not divulged by supplier
LAMP-1	Ab	MM	Lysosomal marker	—	Target sequence not divulged by supplier
ZO-1	BD	MM	Tight junction marker	aa 1048–1247	Target sequence not divulged by supplier

Ab, Abcam; Fg, Fabgennix; BD, BD Biosciences; RP, rabbit polyclonal; MM, mouse monoclonal.