Skip to main content
. 2009 Mar 30;587(Pt 10):2327–2339. doi: 10.1113/jphysiol.2008.167759

Figure 2. Saturation of vesicular glutamate filling at physiological temperature.

Figure 2

A, in simultaneous pre- and postsynaptic whole-cell recordings, qEPSCs were recorded in the presence of TTX (1 μm), with the presynaptic pipette solution containing 10 mm or 100 mm l-glutamate at PT. After obtaining control qEPSCs, kynurenate (100 μm) was bath-applied. Sample average traces (top) and amplitude histograms (bottom) of qEPSCs in the presence (Kyn, red) or absence (Control, black) of kynurenate (superimposed), with 10 mm (left) or 100 mm (right) l-glutamate in the presynaptic pipette solution. The total number of events is 362 for each with 10 mm l-glutamate and 455 for each with 100 mm l-glutamate. B, the percentage of qEPSC inhibition by kynurenate with 10 mm (10 Glu, n= 5) and 100 mm (100 Glu, n= 7) l-glutamate in the nerve terminals. No significant difference was observed (N.S., P > 0.4). C, similar c.v.s of the qEPSC amplitudes in the presence of l-glutamate loaded at 1–100 mm, or without presynaptic whole-cell recording (Control), at PT (left) and RT (right).