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. 2009 Feb 13;33(3-3):299–311. doi: 10.1016/j.molcel.2009.01.019

Figure 5.

Figure 5

Localization of Scm3Sp Is Dependent on Mis6, Sim4, Mis16, Mis18, but Not CENP-ACnp1

(A) Wild-type, cnp1-1, mis6-302, sim4-193, mis16-53, and mis18-262 strains expressing Scm3-GFP were shifted to 36°C for 6 hr, fixed, and processed for immunofluorescence using anti-GFP (Scm3-GFP; green), anti-Cdc11 antibodies (SPB; red), and DAPI (blue). Identical exposures and processing were performed to ensure that wild-type and mutant images are comparable. Representative images are presented. All cells are in G2. Scale bar 5 μm.

(B) ChIP of Scm3-GFP in the indicated strains shifted to 36°C for 6 hr before fixation and analysis by ChIP with anti-Cnp1/CENP-ACnp1 (C) and anti-GFP (G, Scm3-GFP) antibodies. PCR analysis as in Figure 1E. Part of same experiment shown in Figure 3C; wild-type control identical.