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Western blot of phosphorylated IGF-IR (pIGF-IR), IGF-IR, phosphorylated Akt (pAkt), Akt, phosphorylated CREB (pCREB), CREB, and phosphorylated ATF-1 (pATF-1) in wild-type (WT), SPC-IGFIR, or CCSP-IGFIR transgenic mice administered doxycycline for 8 months. Tubulin served as a loading control.
