Table 1.

Sequences of the (A) Two CpG-rich Regions of LHX9 Analyzed and (B) Primers Used for Amplification Reactions.

(A) Sequences of the CpG-rich regions of LHX9 gene showing the positions of CpG sites (cg) in gray and the primers (bold italic) used for amplification after bisulfite treatment of genomic DNA. The BstUI (5′-CGCG-3′) and Taq^αI (5′-TCGA-3′) restriction sites are boxed. (B) Sequences of the primers (bis-) used for amplification of bisulfite-modified genomic DNA and the primers used for the cRT-PCR, which were always chosen to span at least 1 intron. The cRT-PCR was described in the Materials and Methods section using T7-forward primer (italic) and deletion reverse primer. Arrow in the sequence of the deletion reverse primer (DEL) indicates the deletion.