Phylogentic relationships of methionine synthase, SAM synthetase and ACC oxidase genes from N. suaveolens. Unrooted neighbour-joining phylogenetic trees based on amino acid sequence similarities. Trees were created with PAUP (version 4.0b10) and displayed by using TreeView. Gaps in the alignments (Clustal W) were removed for analysis. Bootstrap values below 50% were not included (1,000 replicates). Symbols 1–4 indicate conserved motifs. (A) methionine synthase: Arabidopsis thaliana (1:AAK 64167, 2:AAN 31838), Catharanthus roseus (CAA 58474), Glycine max (AAQ 08403), Hordeum vulgare (1:BAD 34660, 2:BAD 34661), Medicago truncatula (ABE 84165), Nicotiana suaveolens (DQ 984138), Oryza sativa (ABA 99427) Solanum tuberosum (AAF 74983), Zea mays (AAL 33589). (B) SAM synthetase: Actinidia chinensis (1:AAA 81378, 2:P 50302), Arabidopsis thaliana (NP 1811225), Beta vulgaris (BAE 07180), Brassica juncea (AAK 71234), Catharanthus roseus (1:CAA 95856, 2:CAA 95857, 3:CAA 95858), Lycopersicon esculentum (CAA 80867), Nicotiana tabacum (AAF 42974), Nicotiana suaveolens (DQ984137), Petunia hybrida (CAA 57696), Solanum tuberosum (1:ABB 02634, 2:ABB 29942), Sueda salsa var. mariti. (C) ACC oxidase: Actinidia deliciosa (BAA 21541), Arabidopsis thaliana (AC 27484), Caprica papaya (AAL 78058), Lycopersicon esculentum (BAA 34924), Medicago truncatula (AAL 35971), Nicotiana glutinosa (AAA 99793), Nicotiana suaveolens (DQ 984136), Nicotiana tabacum (CAA 58232), Pelargonium hortorum (AAC 48977), Petunia hybrida (1:Q08508, 2:Q08506), Pisum sativum (P 31239), Solanum tuberosum (AAK 68076), Trifolium repens (AAD 28197)