Fig. 3.

RNA expression of methionine synthase, SAM synthetase and ACC oxidase in different organs of N. suaveolens. Plant tissues (petal lobes, stigmas, stamens, petal tubes, sepals, leaves, stems, roots) were harvested at 6 pm from 3-month-old plants. RNA was isolated and 5 μg of total RNA was separated on denaturing agarose gels. RNA was blotted and hybridised with NsSAMS1, NsMS1 and NsACO probes (Fig. S3). Blots were rehybridized with 18S rDNA probe to allow normalization. The quantification was based on two independent experiments and duplicated Northern blots. Highest expression levels were set at 100% and relative transcript levels were calculated, SE is indicated (n = 4). Black column: methionine synthase; grey column: SAM synthetase, light grey column: ACC oxidase