Fig. 4.

RNA expression profile of methionine synthase, SAM synthetase and ACC oxidase of N. suaveolens at different times during the day and during flower development. Plants were grown under 16 h light (L) and 8 h darkness (D; 10 pm to 6 am) (details of growing conditions are given in M and M). Petals of N. suaveolens were harvested at indicated time points at six consecutive days after flower opening. RNA was isolated and 5 μg of total RNA was separated on denaturing agarose gels. RNA was blotted and hybridised with NsSAMS1, NsMS1 and NsACO probes (Fig. S4). Blots were rehybridized with 18S rDNA probe to allow normalisation. The quantification was based on two independent experiments and duplicated Northern blots, highest expression levels were set at 100% and relative transcript levels were calculated, SE is indicated (n = 4). (A) methionine synthase; (B) SAM synthetase; (C) ACC oxidase