Fig. 7.

In vitro effects of SDF-1 on the liver cancer cells. (A) SDF-1 does not induce cellular transmigration. The cells were loaded into the upper chamber of a Transwell and they were allowed to migrate to the lower chamber, which contained 100 ng/ml SDF-1, over a 4-h period. The data is given as the mean±S.D. of the migration index from three independent experiments. (B) SDF-1 does not stimulate cellular proliferation. A modified MTT assay, known as a CCK-8 assay, was used. The data is given as the mean±S.D. of the proliferation index from three independent experiments. (C) SDF-1 does not rescue liver cancer cells from serum deprivation-induced apoptosis. The cells were incubated in serum-free RPMI medium in the presence or absence of 100 ng/ml SDF-1 for 24 h and then they were subjected to flow cytometric analysis.