Table 2.

Effect of 10 mM saccharolactone on the hydrolysis of estradiol 3-glucuronide (E-3-G, 100 μM) and estradiol 17-glucuronide (E-17-G, 200 μM). Estradiol glucuronide concentrations were measured by HPLC before and after 6 hour incubation with human liver, intestine, kidney, and lung microsomes, insect cell expressed UGTs, and control cells.

Glucuronide	Saccharolactone	pHLM	Intestine	Kidney	Lung	UGT2B7	UGT1A1	Insect control	HEK293control	Buffer
E-3-G	−	82±1a,b	104±3	104±1a,b	85±6	77±9	77±2	90±5	102±4	101±5
	+	84±2a	101±3	110±3	80.9±5a	91±5	87±7	89±7	98±10	95±4
E-17-G	−	86±2b	110±4b	104±3	92±6	75±5	71±4	88±3	104±3	102±13
	+	84±3	106±2	116±2	86±3	89±5	85±3	88±4	101±8	95±3

Data are presented as mean±s.d. of percent glucuronide remaining after 6 hours, n=3.

^aP<0.01 comparing 0 hr timepoint vs. 6 hr timepoint.

^bP<0.05 comparing 6 hour timepoint with and without saccharolactone by unpaired t-test.