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. 2000 Jul 11;97(15):8490–8494. doi: 10.1073/pnas.150104097

Figure 2.

Figure 2

(A) Repaired p53 RNAs generated in Saos-2 cells. Cells were transfected with a truncated p53 expression plasmid (pC53 5′ trunc; lanes 1 and 3–5) alone (lane 1) or with the active (pRib41p53, lanes 2 and 3; lane 5 mix sample) or with the inactive (pdRib41p53, lane 4) ribozyme expression plasmid. Corrected p53 RNAs were amplified by RT-PCR, yielding a DNA fragment of 137 bp. The migration of size markers of 134, 154, and 201 bp is indicated. (B) Sequences of amended p53 transcripts produced in Saos-2 cells. Sequences of two representative clones are shown. The expected sequence for a corrected transcript around the splicing junction is shown along with the predicted translation product. The ribozyme recognition sequence is boxed, and the nucleotide at the position 41 is outlined.