Figure 3.

p53-mediated transcriptional transactivation after RNA repair. (A) Saos-2 cells were transfected with the PG13-luc reporter construct and the empty parental expression vectors (Vectors), the truncated 5′ p53 expression plasmid alone (pC53 5′ trunc), each ribozyme expression plasmid alone [pRib41p53 (R41) or pRib65p53 (R65)], or pC53 5′ trunc along with pRib41p53 or pRib65p53. (B) SW480 cells were transfected with the PG13-luc and each ribozyme expression vector (pRib41p53 and pRib65p53) or an empty vector. Relative luciferase activity was quantitated as a percentage of the vector control sample, and average values ± SD for luciferase activity from three independent experiments are shown.