Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1991 Feb;29(2):291–296. doi: 10.1128/jcm.29.2.291-296.1991

Characterization of noncapsulate Haemophilus influenzae by whole-cell polypeptide profiles, restriction endonuclease analysis, and rRNA gene restriction patterns.

K D Bruce 1, J Z Jordens 1
PMCID: PMC269756  PMID: 1706727

Abstract

Thirty-four clinical isolates of noncapsulate Haemophilus influenzae representing isolates with either related or dissimilar patterns of whole-cell polypeptide profiles on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) were further characterized by restriction enzyme analysis (REA) and rRNA gene restriction patterns. Total cellular DNA was extracted by a rapid, microcentrifuge-scale method and digested with BamHI, which gave a pattern of about 18 discrete bands. This confirmed the five closely related groupings suggested by SDS-PAGE. Isolates dissimilar by SDS-PAGE were also distinguishable by REA. However, there was no correlation between the degrees of similarity estimated from whole-cell polypeptide profiles and those obtained from REA for the dissimilar isolates. Therefore, inferences of genetic relatedness made on the basis of these data should be interpreted with caution. rRNA gene restriction patterns also confirmed the groupings suggested by the other two techniques. We conclude that the three methods were highly discriminatory and that whole-cell polypeptide patterns or REA with BamHI would be appropriate techniques for epidemiological studies of noncapsulate H. influenzae.

Full text

PDF
291

Images in this article

293Image
on p.293
293Image
on p.293
295Image
on p.295

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barton L. L., Granoff D. M., Barenkamp S. J. Nosocomial spread of Haemophilus influenzae type b infection documented by outer membrane protein subtype analysis. J Pediatr. 1983 Jun;102(6):820–824. doi: 10.1016/s0022-3476(83)80005-5. [DOI] [PubMed] [Google Scholar]
  2. Coverdale C. H., Temple G. S. Outer membrane protein and biotype analysis of non-serotypable strains of Haemophilus influenzae. J Clin Pathol. 1989 Apr;42(4):409–413. doi: 10.1136/jcp.42.4.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Everett E. D., Rham A. E., Jr, Adaniya R., Stevens D. L., McNitt T. R. Haemophilus influenzae pneumonia in adults. JAMA. 1977 Jul 25;238(4):319–321. [PubMed] [Google Scholar]
  4. Grimont F., Grimont P. A. Ribosomal ribonucleic acid gene restriction patterns as potential taxonomic tools. Ann Inst Pasteur Microbiol. 1986 Sep-Oct;137B(2):165–175. doi: 10.1016/s0769-2609(86)80105-3. [DOI] [PubMed] [Google Scholar]
  5. Groeneveld K., van Alphen L., Eijk P. P., Jansen H. M., Zanen H. C. Changes in outer membrane proteins of nontypable Haemophilus influenzae in patients with chronic obstructive pulmonary disease. J Infect Dis. 1988 Aug;158(2):360–365. doi: 10.1093/infdis/158.2.360. [DOI] [PubMed] [Google Scholar]
  6. Inzana T. J. Electrophoretic heterogeneity and interstrain variation of the lipopolysaccharide of Haemophilus influenzae. J Infect Dis. 1983 Sep;148(3):492–499. doi: 10.1093/infdis/148.3.492. [DOI] [PubMed] [Google Scholar]
  7. Irino K., Grimont F., Casin I., Grimont P. A. rRNA gene restriction patterns of Haemophilus influenzae biogroup aegyptius strains associated with Brazilian purpuric fever. J Clin Microbiol. 1988 Aug;26(8):1535–1538. doi: 10.1128/jcm.26.8.1535-1538.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jordens J. Z., Hall L. M. Characterisation of methicillin-resistant Staphylococcus aureus isolates by restriction endonuclease digestion of chromosomal DNA. J Med Microbiol. 1988 Oct;27(2):117–123. doi: 10.1099/00222615-27-2-117. [DOI] [PubMed] [Google Scholar]
  9. Kilian M. A taxonomic study of the genus Haemophilus, with the proposal of a new species. J Gen Microbiol. 1976 Mar;93(1):9–62. doi: 10.1099/00221287-93-1-9. [DOI] [PubMed] [Google Scholar]
  10. Loos B. G., Bernstein J. M., Dryja D. M., Murphy T. F., Dickinson D. P. Determination of the epidemiology and transmission of nontypable Haemophilus influenzae in children with otitis media by comparison of total genomic DNA restriction fingerprints. Infect Immun. 1989 Sep;57(9):2751–2757. doi: 10.1128/iai.57.9.2751-2757.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Maggs A. F., Pennington T. H. Temporal study of staphylococcal species on the skin of human subjects in isolation and clonal analysis of Staphylococcus capitis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. J Clin Microbiol. 1989 Dec;27(12):2627–2632. doi: 10.1128/jcm.27.12.2627-2632.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Murphy T. F., Apicella M. A. Antigenic heterogeneity of outer membrane proteins of nontypable Haemophilus influenzae is a basis for a serotyping system. Infect Immun. 1985 Oct;50(1):15–21. doi: 10.1128/iai.50.1.15-21.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Murphy T. F., Dudas K. C., Mylotte J. M., Apicella M. A. A subtyping system for nontypable Haemophilus influenzae based on outer-membrane proteins. J Infect Dis. 1983 May;147(5):838–846. doi: 10.1093/infdis/147.5.838. [DOI] [PubMed] [Google Scholar]
  14. Musser J. M., Granoff D. M., Pattison P. E., Selander R. K. A population genetic framework for the study of invasive diseases caused by serotype b strains of Haemophilus influenzae. Proc Natl Acad Sci U S A. 1985 Aug;82(15):5078–5082. doi: 10.1073/pnas.82.15.5078. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Olyhoek T., Crowe B. A., Wall R. A., Achtman M. Comparison of clonal analysis and DNA restriction analysis for typing of Neisseria meningitidis. Microb Pathog. 1988 Jan;4(1):45–51. doi: 10.1016/0882-4010(88)90047-2. [DOI] [PubMed] [Google Scholar]
  16. Owen R. J. Chromosomal DNA fingerprinting--a new method of species and strain identification applicable to microbial pathogens. J Med Microbiol. 1989 Oct;30(2):89–99. doi: 10.1099/00222615-30-2-89. [DOI] [PubMed] [Google Scholar]
  17. Paterson A. J., Macsween K. F., Pennington T. H. Haemophilus influenzae subtyping by SDS-PAGE of whole-cell polypeptides. Epidemiol Infect. 1987 Aug;99(1):179–189. doi: 10.1017/s0950268800067017. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Pennington T. H., Freebairn E. M. Subtyping of Haemophilus influenzae type b strains from Europe and North America by SDS-PAGE of whole-cell polypeptides: the geographical distribution of subtypes. Epidemiol Infect. 1989 Feb;102(1):11–19. doi: 10.1017/s0950268800029630. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Porras O., Caugant D. A., Gray B., Lagergård T., Levin B. R., Svanborg-Edén C. Difference in structure between type b and nontypable Haemophilus influenzae populations. Infect Immun. 1986 Jul;53(1):79–89. doi: 10.1128/iai.53.1.79-89.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Salvi R. J., Ahroon W., Saunders S. S., Arnold S. A. Evoked potentials: computer-automated threshold-tracking procedure using an objective detection criterion. Ear Hear. 1987 Jun;8(3):151–156. [PubMed] [Google Scholar]
  21. Spagnuolo P. J., Ellner J. J., Lerner P. I., McHenry M. C., Flatauer F., Rosenberg P., Rosenthal M. S. Haemophilus influenzae meningitis: the spectrum of disease in adults. Medicine (Baltimore) 1982 Mar;61(2):74–85. [PubMed] [Google Scholar]
  22. Sturm A. W., Mostert R., Rouing P. J., van Klingeren B., van Alphen L. Outbreak of multiresistant non-encapsulated Haemophilus influenzae infections in a pulmonary rehabilitation centre. Lancet. 1990 Jan 27;335(8683):214–216. doi: 10.1016/0140-6736(90)90291-c. [DOI] [PubMed] [Google Scholar]
  23. Trollfors B. Two cases of septicemia caused by noncapsulated strains of Haemophilus influenzae. Scand J Infect Dis. 1978;10(3):247–248. doi: 10.3109/inf.1978.10.issue-3.16. [DOI] [PubMed] [Google Scholar]
  24. Wald E. R. Haemophilus influenzae as a cause of acute otitis media. Pediatr Infect Dis J. 1989 Jan;8(1 Suppl):S28–S30. [PubMed] [Google Scholar]
  25. Weinberg G. A., Ghafoor A., Ishaq Z., Nomani N. K., Kabeer M., Anwar F., Burney M. I., Qureshi A. W., Musser J. M., Selander R. K. Clonal analysis of Hemophilus influenzae isolated from children from Pakistan with lower respiratory tract infections. J Infect Dis. 1989 Oct;160(4):634–643. doi: 10.1093/infdis/160.4.634. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES