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Size-exclusion HPLC analysis of 64Cu-radiolabeled anti-CEA minibody. (A) Analysis and purification of radiolabeled minibody; peak fractions were pooled for animal studies. (B) Upper trace, starting sample used for evaluation of immunoreactivity. Lower trace, after incubation with CEA the bulk of the 64Cu-minibody is found in antibody/antigen complexes.
