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. Author manuscript; available in PMC: 2009 Jun 16.
Published in final edited form as: J Steroid Biochem Mol Biol. 2007 Jun 26;107(3-5):202–210. doi: 10.1016/j.jsbmb.2007.03.046

Figure 2.

Figure 2

Sulfation and desulfation of Tib and metabolites. The formation of Tib and the Δ4 monosulfates was carried using SULT2A1 and the 3α- and 3β-monosulfates were generated using SULT1E1 in the presence of 35S-PAPS. The four monosulfates were then tested for sensitivity for hydrolysis by STS. Panel A is the autoradiograph of the formation and hydrolysis of the four monosulfates. Panel B shows the selective hydrolysis of Tib 3β-monosulfate by STS. Each reaction was run in quadruplicate and the mean ± s.d. of the 35S-Tib metabolites generated or remaining after STS incubation is shown.