Skip to main content
. Author manuscript; available in PMC: 2009 Jun 16.
Published in final edited form as: J Steroid Biochem Mol Biol. 2007 Jun 26;107(3-5):202–210. doi: 10.1016/j.jsbmb.2007.03.046

Figure 3.

Figure 3

Hydrolysis of the 3α- and 3β-Tib mono- and disulfates by STS. The 3α- and 3β-Tib monosulfates and disulfates were synthesized using SULT1E1 and 35S-PAPS. The levels of the synthesized sulfates were quantified by resolution of an aliquot of the reaction by TLC, autoradiography and scintillation spectroscopy. Aliquots of the reactions were then extracted with chloroform and the Tib sulfates used as substrates for STS. After 60 min incubations the reactions were resolved by TLC and the Tib sulfates quantified by scintillation spectroscopy. The mean ± s.d. of four reactions is shown. An asterisk (*) indicates a significant difference between the assays with and without STS (p<0.01).