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. Author manuscript; available in PMC: 2009 Jun 16.
Published in final edited form as: J Steroid Biochem Mol Biol. 2007 Jun 26;107(3-5):202–210. doi: 10.1016/j.jsbmb.2007.03.046

Figure 4.

Figure 4

Time course of tibolone monosulfate and disulfate hydrolysis by STS. The tibolone monosulfates and disulfate were generated using SULT2A1 and 10 μM 3α-OH or 3β-OH tibolone and 35S-PAPS. The 3-OH tibolone sulfates were extracted with chloroform and hydrolyzed with STS as described in Methods. Panel A shows the results of the hydrolysis of the mono- and disulfates formed with 3α-OH tibolone by STS. Panel B shows the results of the hydrolysis of the 3β-OH tibolone mono- and disulfates by STS. Aliqouts were taken from the STS reactions at different times, resolved by TLC and quantified. The mean ± s.d. of four reactions is shown.