Skip to main content
. Author manuscript; available in PMC: 2009 Jun 16.
Published in final edited form as: J Steroid Biochem Mol Biol. 2007 Jun 26;107(3-5):202–210. doi: 10.1016/j.jsbmb.2007.03.046

Figure 5.

Figure 5

Sulfation and desulfation of raloxifene. Raloxifene monosulfate was formed using SULT2A1 and both raloxifene monosulfates and disulfate were synthesized using SULT1E1 and 35S-PAPS. As reported previously (12), panel A shows that using SULT1E1, raloxifene disulfate formation at 2.5 μM is favored over monosulfate synthesis although monosulfate formation is greater at the 10 μM concentration. Panel B shows the sensitivity of the raloxifene monosulfates and disulfate for sensitivity to hydrolysis by STS. The products of the STS reactions were resolved by TLC and the products quantified by scintillation spectroscopy Each reaction was run in quadruplicate and the mean ± s.d. of the 35S-raloxifene sulfates generated or remaining after STS incubation is shown.