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HPLC analysis of the hydrolysis of the raloxifene monosulfates and disulfate by human placental STS. Raloxifene monosulfates and disulfate were synthesized using SULT2A1 and 1E1 as described in Figure 6. Panel A shows the elution of the monosulfates formed by SULT1E1 before and after STS hydrolysis. Panel B shows the elution of the monosulfate formed by SULT2A1 before and after STS hydrolysis.
