Figure 8.

Genetic interactions between mbc, elmo, and Rac alleles. (A–D, F, G) Scanning electron micrographs of adult eyes expressing UAS-cDNAs under control of GMR-GAL4. (A, B) Overexpression of mbc or elmo has no apparent impact on eye morphology. (C) Co-expression of mbc and elmo results in a severe rough eye phenotype. (D) Removal of one copy of Rac1^J11Rac2^Δ suppresses the rough eye phenotype, resulting in nearly wild-type eye morphology. (E) Graph depicting the ratio of abnormal:normal (A:N) ommatidia present in flies co-expressing mbc and elmo compared to flies co-expressing mbc and elmo in a heterozygous background for mbc, elmo, or Rac1Rac2. Adult eyes co-expressing mbc and elmo (white bar) exhibit a ratio of 25:1 (n=32) while the ratio of abnormal:normal ommatidia decreases when heterozygous for mbc^D11.2 (A:N=0.65; n=20), elmo^19F3 (A:N=0.51; n=20) or Rac1^J11Rac2^Δ (A:N=1.32; n=48). n is the number of eyes examined for each genotype. See also Supp Fig. 2(F, G) Expression of RacN17 results in a severe rough eye morphology (F) that is suppressed by coincident expression of mbc and elmo (G). Scale bar, 200μM