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. Author manuscript; available in PMC: 2009 Jun 17.
Published in final edited form as: Exp Eye Res. 2007 Oct 2;86(1):81–91. doi: 10.1016/j.exer.2007.09.011

Figure 5. Quantitation of Kir7.1 and Kir7.1S mRNA levels in native human RPE.

Figure 5

(A) Amplification plots of Kir7.1 (green), Kir7.1S (red) and GAPDH (dark blue) in native human RPE. The y-axis represents the fluorescence intensity and the x-axis the PCR cycle number. (B) Melting curves of Kir7.1 (green), Kir7.1S (red) and GAPDH (dark blue). (C) Agarose gel electrophoresis of the expression of Kir7.1, Kir7.1S and GAPDH in native human RPE. (D) Summary of the relative expression of Kir7.1 and Kir7.1S relative to GAPDH in the RPE obtained from the five donors. *P < 0.01 compared to Kir7.1.