Table 3.
Fluorescence properties of AT variants with and without the pentasaccharide.
| % Enhancement | ||||||
|---|---|---|---|---|---|---|
| AT | H5 | λmax (nm) | F at λmax | Δλmax (nm) | at λmax | at 340nm |
| S137A | − | 340.25 | 213.6 | +0.5 | 38.9 | 38.9 |
| + | 340.75 | 296.7 | ||||
| V375C | − | 341.25 | 223.4 | −1.0 | 37.8 | 38.0 |
| + | 340.25 | 307.9 | ||||
| S380C | − | 340.75 | 229.3 | −0.75 | 13.0 | 13.0 |
| + | 340 | 259.0 | ||||
| V375C/S380C | − | 344.5 | 198.3 | −2.75 | 2.9 | 3.6 |
| + | 341.75 | 204.1 | ||||
F is the fluorescence intensity.