Abstract
A BACTEC aerobic nonradiometric medium, PEDS Plus, designed for diagnosis of pediatric bacteremia was evaluated in three hospital centers. Equivalent blood volumes (up to 5 ml) were inoculated into and incubated in BACTEC NR-6A (6A) and PEDS Plus broths. Among 4,581 compliant sets, 289 clinically significant organisms, representing more than 20 bacterial and two Candida species, were isolated. One hundred eighty-one isolates were recovered in both bottles, 75 in PEDS Plus only, and 33 in 6A only (P less than 0.001). Time to detection when both bottles were positive was the same for 129 isolates, detection with PEDS Plus was earlier for 39, and detection with 6A was earlier for 13 (P less than 0.005). Staphylococcus aureus was recovered significantly more often in PEDS Plus than in 6A (P less than 0.01), and more coagulase-negative staphylococci and pediatric pathogens (pneumococci, Haemophilus influenzae, and Streptococcus agalactiae) were recovered in PEDS Plus than in 6A. Coagulase-negative staphylococci and H. influenzae were detected significantly earlier in PEDS Plus (P less than 0.05 and less than 0.01, respectively). When the eight species of the family Enterobacteriaceae isolated were considered together, recovery in PEDS Plus was better than in 6A (P less than 0.05). For 66 of the 143 isolates from patients known to be on antimicrobial therapy at the time blood was drawn, PEDS Plus was superior to 6A. In 45 cases, organisms were isolated from PEDS Plus only (P less than 0.001) and in 21 cases they were isolated from PEDS Plus before 6A (P less than 0.01). PEDS Plus broth aids diagnosis of pediatric bacteremia by increasing recovery of etiologic agents and decreasing the time required for detection.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Appelbaum P. C., Beckwith D. G., Dipersio J. R., Dyke J. W., Salventi J. F., Stone L. L. Enhanced detection of bacteremia with a new BACTEC resin blood culture medium. J Clin Microbiol. 1983 Jan;17(1):48–51. doi: 10.1128/jcm.17.1.48-51.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Campos J. M. Detection of bloodstream infections in children. Eur J Clin Microbiol Infect Dis. 1989 Sep;8(9):815–824. doi: 10.1007/BF02185854. [DOI] [PubMed] [Google Scholar]
- Courcol R. J., Durocher A. V., Roussel-Delvallez M., Fruchart A., Martin G. R. Routine evaluation of BACTEC NR-16A and NR-17A media. J Clin Microbiol. 1988 Sep;26(9):1619–1622. doi: 10.1128/jcm.26.9.1619-1622.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Edberg S. C., Bottenbley C. J., Gam K. Use of sodium polyanethol sulfonate to selectively inhibit aminoglycoside and polymyxin antibiotics in a rapid blood level antibiotic assay. Antimicrob Agents Chemother. 1976 Mar;9(3):414–417. doi: 10.1128/aac.9.3.414. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Eng J., Holten E. Gelatin neutralization of the inhibitory effect of sodium polyanethol sulfonate on Neisseria meningitidis in blood culture media. J Clin Microbiol. 1977 Jul;6(1):1–3. doi: 10.1128/jcm.6.1.1-3.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Favor L. F., Tarpay M., Blackstock R. Septicemia in children with cancer. South Med J. 1979 Feb;72(2):132–135. doi: 10.1097/00007611-197902000-00007. [DOI] [PubMed] [Google Scholar]
- Kennaugh J. K., Gregory W. W., Powell K. R., Hendley J. O. The effect of dilution during culture on detection of low concentrations of bacteria in blood. Pediatr Infect Dis. 1984 Jul-Aug;3(4):317–318. doi: 10.1097/00006454-198407000-00008. [DOI] [PubMed] [Google Scholar]
- Noel G. J., Laufer D. A., Edelson P. J. Anaerobic bacteremia in a neonatal intensive care unit: an eighteen-year experience. Pediatr Infect Dis J. 1988 Dec;7(12):858–862. [PubMed] [Google Scholar]
- Strand C. L., Hickman A., Bryant J. K., Sutton K. Evaluation of the BACTEC resin blood-culture medium. Am J Clin Pathol. 1983 Nov;80(5):706–710. doi: 10.1093/ajcp/80.5.706. [DOI] [PubMed] [Google Scholar]
- Stratton C. W., Weinstein M. P., Mirrett S., Paisley J., Lauer B. A., Reller L. B. Controlled evaluation of blood culture medium containing gelatin and V-factor-analog for detection of septicemia in children. J Clin Microbiol. 1988 Apr;26(4):747–749. doi: 10.1128/jcm.26.4.747-749.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Washington J. A., 2nd, Ilstrup D. M. Blood cultures: issues and controversies. Rev Infect Dis. 1986 Sep-Oct;8(5):792–802. doi: 10.1093/clinids/8.5.792. [DOI] [PubMed] [Google Scholar]