Figure 3.

Voltage-gated Na⁺ channel proteins in Mat-LyLu rat prostate cancer cell lines. (A) Expression of genes for voltage-gated Na⁺ channels, detected by reverse transcriptase polymerase chain reaction (RT-PCR) in Mat-LyLu cells. M, Marker. (B) Summary results of real-time quantitative RT-PCR. The expression levels of SCN channel genes were normalized to those of the 18S ribosomal RNA levels. Data are means ± SEM from six independent samples. (C) Immunocytochemical detection with PanNa_V in Mat-LyLu cells. Staining with Hoechst 33258 to visualize nuclei (Ca), PanNa_V (Cb), negative control (without antibody, Cc), double staining with Hoechst 33258 and PanNa_V (Cd).