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. 2009 Mar 9;156(7):1136–1146. doi: 10.1111/j.1476-5381.2009.00123.x

Figure 7.

Figure 7

Effects of Rg1 on serine-phosphorylation of MEK in human embryonic kidney (HEK293) cells. (A) HEK293 cells were treated with 50 ng·mL−1 IGF-1 and 1 pmol·L−1 Rg1 for varying lengths of time (0, 1, 3, 5, 10 and 30 min). (B) HEK293 cells were treated with 10−14 (R14), 10−12 (R12), 10−10 (R10), 10−8 (R8), and 10−6 mol·L−1 (R6) of Rg1 for 3 min. Proteins were isolated and fractionated using 10% SDS-PAGE and immunoblotted with pMEK and MEK antibody. The signals were detected and quantified by the Lumi-Imager. Results were obtained from three independent experiments and expressed as mean ± SEM. *P < 0.05 versus control, n = 3.