. 2009 Feb 17;156(7):1147–1153. doi: 10.1111/j.1476-5381.2009.00113.x

Table 2.

Competitive inhibition by darifenacin of specific [N-methyl-³H]scopolamine methyl chloride ([³H]NMS) binding in the submandibular gland and bladder of wild type (WT), M₂ and M₃ knockout (KO) mice

	Submandibular gland		Bladder		K_i (submandibular gland)/K_i (bladder)
	K_i (nmol·L⁻¹)	nH	K_i (nmol·L⁻¹)	nH
WT	3.0 ± 0.6^*	0.8 ± 0.1	24 ± 7	0.6 ± 0.1	0.1
M₂KO	4.3 ± 0.9^##	1.0 ± 0.1^†	2.5 ± 0.7†^,^#	1.0 ± 0.2^†	1.7
M₃KO	19 ± 2*^,^††	1.1 ± 0.1^†	43 ± 10^†	0.9 ± 0.1^†	0.4

The submandibular gland and bladder were taken from WT and muscarinic acetylcholine receptor (M₂, M₃) KO mice. Specific [³H]NMS binding in the submandibular gland (0.1 nmol·L⁻¹) and bladder (0.2 nmol·L⁻¹) was measured in the absence and presence of five to six different concentrations (0.3 nmol·L⁻¹–3 µmol·L⁻¹) of darifenacin, and the values of K_i and Hill coefficients (nH) were estimated. Values represent the mean ± SEM of three to six determinations.

Symbols show a significant difference from the values for bladder in the same group of mice

(P < 0.05), a significant difference from the values for the same tissue in WT mice

^†

(P < 0.05,

^††

P < 0.001) and a significant difference from the values for the same tissue in M₃KO mice

(P < 0.05,

^##

P < 0.001).