Figure 7.

T-bet is essential for CAME-driven IFNγ suppression. CD4+ Th cells were isolated from the lymph nodes of DTg-KO mice and activated for 24 h. Cells were treated with CAME in the presence or absence of doxycycline (Dox; 0.1 µg·mL⁻¹). Whole cell extracts were prepared from the cells and analysed by immunoblot analysis (A); results shown are representative of three independent experiments. Cell supernatants were used for measuring IFNγ (B) and IL-2 (C) by elisa; data are given as means ± SD; n= 3. #P < 0.05; *P < 0.05; unpaired t-test. CAME, p-coumaryl alcohol-γ-O-methyl ether; DTg-KO, double transgenic-knockout; elisa, enzyme-linked immunosorbent assay; IFNγ, interferon-γ; IL-2, interleukin-2; T-bet, T-box protein expressed in T cells; Th cell, T helper cell.