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. 2000 Jul 18;97(15):8501–8506. doi: 10.1073/pnas.97.15.8501

Figure 2.

Figure 2

(A) Endogenous transcriptional activation of the p53 responsive promoter PG13 and MG15 containing mutated binding site for p53. The values represent relative activities of PG13 versus MG15 construct. SiHa DD is a SiHa cell line stably transfected with a dominant negative p53 gene. (B) Transcriptional activation of p53 responsive promoter after treatment with LMB and AD. Twenty-four hours after the transfection, cell lines were treated with the drugs and thereafter analyzed for luciferase activity. The bars represent: 1, 20 nM LMB; 2, 200 nM LMB; 3, 5 nM AD; 4, 50 nM AD; 5, 20 nM LMB + 5 nM AD; 6, untreated cells. Values are expressed relative to the readings of untreated cells arbitrarily set to 1. SiHa DD values are calculated relative to untreated SiHa CMV cells. * and **, not enough living cells for reliable quantitation. SD was calculated from triplicate wells.