Table 1.

The Genbank accession numbers, primer sequences and amplicon sizes for GR, ERα and ERβ

Gene	Accession no.	Forward	Reverse	Amplicon size
GR	NM_001018077	TGGTTCCGAAAATTGGAATA	AAGCTTCATCAGAGCACACC	209 bp
ERα	NM_000125	AACACAAGCGCCAGAGAGAT	AGGATCTCTAGCCAGGCACA	364 bp
ERβ	NM_001437	CGAAGTGGGAATGGTGAAGT	ACAAAGCCGGGAATCTTCTT	330 bp

These primers were all used in RT-PCR experiments at an annealing temperature of 58°C for 35 cycles to confirm expression of the genes in HUVECs.

ER, oestrogen receptor; GR, glucocorticoid receptor; HUVECs, human umbilical vein endothelial cells; RT, reverse transcriptase.