Figure 4.

Effects of myrtucommulone (MC) on the formation of prostaglandin (PG)E₂ and 6-oxo PGF_1α in intact A549 cells. (A) PGE₂ formation. A549 cells (4 × 10⁶ per·mL) were pre-incubated with MK-886, MC or vehicle (dimethyl sulphoxide, DMSO, w/o) for 10 min, then cellular PGE₂ synthesis was elicited by addition of 2.5 µmol·L⁻¹ A23187 plus 1 µmol·L⁻¹ arachidonic acid and [³H]arachidonic acid (18.4 kBq). After 15 min at 37°C, formed [³H]PGE₂ was analysed by RP-HPLC and liquid scintillation counting as described in the Methods section. (B) 6-oxo PGF_1α formation. Interleukin-1β-stimulated A549 cells (5 × 10⁶ per·mL) were pre-incubated with the indicated concentrations of MC, with MK-886 (30 µmol·L⁻¹), indomethacin (Indo, 10 µmol·L⁻¹), celecoxib (Cele, 5 µmol·L⁻¹) or vehicle (DMSO, w/o) for 15 min prior to addition of 30 µmol·L⁻¹ arachidonic acid. After 15 min at 37°C the amount of 6-oxo PGF_1α was assessed by enzyme-linked immunosorbent assay as described in the Methods section. Data are given as mean ± SE, n = 3, **P < 0.01 or ***P < 0.001 vs. vehicle (0.1% DMSO) control, anova + Tukey HSD post hoc tests.