Figure 5.

Inhibition of Ca_V3.1 by N-arachidonoyl dopamine (NADA) and N-arachidonoyl glycine (NAGly) was voltage- but not use-dependent. Whole-cell patch clamp recordings were made from human Ca_V3.1 channels stably expressed in HEK 293 cells. (B) The time course of inhibition of Ca_V3.1 currents by NADA (300 nmol·L⁻¹) and NAGly (10 µmol·L⁻¹) were similar when currents were evoked by stepping from −86 mV to −26 mV at 1 Hz or 0.05 Hz. (B) The inhibition of Ca_V3.1 by NADA (300 nmol·L⁻¹) and NAGly (10 µmol·L⁻¹) was significantly less when cells were voltage clamped at −126 mV and stepped to −26 mV than when cells were stepped to −26 mV from a holding potential of −86 mV. **P < 0.01 versus −86 mV, Student's t-test.