Table 1.

The effects of NADA and NAGly on the kinetics of Ca_V3 channels

CaV channel	Time to peak (ms)			Deactivation τ (ms)
	Control	NADA	NAGly	Control	NADA	NAGly
3.1	7.2 ± 0.3	6.2 ± 0.3	7.3 ± 0.3	3.2 ± 0.1	3.1 ± 0.1	3.2 ± 0.2
3.2	11.2 ± 0.4	11.5 ± 0.6	11.6 ± 0.4	3.1 ± 0.2	3.1 ± 0.2	3.1 ± 0.2
3.3	43 ± 1	44 ± 2	43 ± 2	2.4 ± 0.1	2.4 ± 0.2	2.3 ± 0.1

Cells expressing recombinant Ca_V3 channels were voltage clamped at −86 mV and then stepped to −26 mV. For examples of these experiments see Figures 2 and 3. The time to peak was measured directly and the decay of the current following repolarization of the membrane to −86 mV fit with a single exponential function. The concentration of NADA was 300 nmol·L⁻¹ for Ca_V3.1 and Ca_V3.3 and 1 µmol·L⁻¹ for Ca_V3.2. 10 µmol·L⁻¹ NAGly was used for each channel. There were no differences in time to peak or deactivation for any current with either drug (paired t-test vs. predrug values for each cell). n = 6–8 for each condition.

NADA, N-arachidonoyl dopamine; NAGly, N-arachidonoyl glycine.