Table 2.

The effects of NADA, NAGly and anandamide (AEA) on the parameters of steady-state activation and inactivation of Ca_V3 channels

Drug	CaV channel	Channel V0.5 (mV)
		Activation	Inactivation
300 nmol·L−1 NADA	3.1	−3 ± 0.3	−11 ± 1**
1 µmol·L−1 NADA	3.2	−2.5 ± 2	−10 ± 3**
300 nmol·L−1 NADA	3.3	−1 ± 0.3	−10 ± 1**
10 µmol·L−1 NAGly	3.1	1 ± 1	−8.5 ± 1**
300 nmol·L−1 AEA	3.1	−2.7 ± 0.5	ND
No drug	3.1	−2 ± 1	−2 ± 3
No drug	3.2	1 ± 2	−2 ± 2
No drug	3.3	−2 ± 2	−2 ± 2

Cells expressing recombinant Ca_V3 channels were voltage clamped at −106 mV and then stepped to potentials above −86 mV (activation) or stepped for 5 s to potentials between −126 and −36 mV before stepping to the test potential of −26 mV. The resulting peak currents were fitted to a Boltzmann equation. Changes in the voltage for half activation/inactivation (V_0.5) of the curves are reported. The “No drug” values represent time-dependent changes under our recording conditions. Curves for NADA and NAGly are illustrated in Figures 7 and 8. NADA, N-arachidonoyl dopamine; NAGly, N-arachidonoyl glycine; ND, not determined.

*P < 0.05,

^**P < 0.01 from control.