Figure 9.

c-fos inhibition and cisplatin resistance in PC Cl3 cells. Cells pretreated with siRNA-c-fos were treated with cisplatin. (A) lysates were separated by 10% SDS-PAGE and analysed by Western blotting using the antibody against c-fos. Sequential incubation of the sheet with anti β-actin antibody confirmed the equal protein loading. (B) Viable cell number was determined 24 h later by the MTT assay. The data are means ± SD of four different experiments run in eight replicates and are presented as percent of control. Values with shared letters are not significantly different according to Bonferroni/Dunne post hoc tests. MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenol tetrazolium bromide; PAGE, polyacrylamide gel electrophoresis; SDS, sodium dodecyl sulphate; siRNA, small interfering RNA.