Figure 3.

Effect of prostaglandin E₂ (PGE₂) and ONO-AE-248 on [Ca²⁺]_i. (A) Liver myofibroblasts cultured on glass coverslips were stimulated with PGE₂ and ONO-AE-248 (1 or 10 µmol·L⁻¹), or ATP (10 µmol·L⁻¹). Changes in [Ca²⁺]_i were measured using Ca²⁺ fluorescent dye fura-2. (B) The area under [Ca²⁺]_i-time curve after stimulation (AUC: 0–2 min). Data are presented as mean ± SEM of 52–110 cells from four separate experiments. **P < 0.01 compared with 10 µmol·L⁻¹ PGE₂-treated cells. AUC, area under curve.