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. 2009 Feb 23;156(5):835–845. doi: 10.1111/j.1476-5381.2008.00051.x

Figure 6.

Figure 6

Effect of prostaglandin E2 (PGE2) on protein kinase C (PKC) phosphorylations. Cell homogenates were analysed by Western blotting after stimulation with: (A, B) 1 µmol·L−1 PGE2 (5 or 15 min) or 1 µmol·L−1 PDBu (5 min), (C, D) 30 min pretreatment with Ro 31-8425 or rottlerin followed by 1 µmol·L−1 PGE2 (15 min). Anti-phospho-PKCδThr505 antibody (A, C) or anti-phospho-PKCεSer729 (B, D) antibody was used. The phosphorylation was measured by densitometry and normalized as described in Methods. Representative pictures are also shown. Data are presented as mean ± SEM. *P < 0.05, **P < 0.01 compared with basal control.