Figure 5.

Effects of montelukast (0.25–2 µmol·L⁻¹) on the influx of ⁴⁵Ca²⁺ into the neutrophils activated with either N-formyl-L-methionyl-L-leucyl-L-phenylalanine (FMLP) (1 µmol·L⁻¹, upper graph) or platelet-activating factor (PAF) (200 nmol·L⁻¹, lower graph). The results are expressed as the mean percentages of the drug-free control systems and vertical lines show SEM (n = 4–8 with two to four replicates for each drug concentration and control system). The absolute values for uptake of ⁴⁵Ca²⁺ by unstimulated neutrophils and for cells activated with FMLP or PAF were 47 ± 25, 150 ± 34 and 148 ± 14 pmol ⁴⁵Ca²⁺ 10⁷ cells⁻¹ respectively. *P < 0.05 to P < 0.01 for comparison with the corresponding chemoattractant-activated montelukast-free control systems (according to the repeated measures anova, there were significant effects of montelukast at both 1 and 2 µmol·L⁻¹ for the FMLP system; on post hoc testing significance remained at 2 µmol·L⁻¹).