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. 2000 Jul 18;97(15):8507–8512. doi: 10.1073/pnas.97.15.8507

Figure 1.

Figure 1

Loss of elongin binding leads to increased proteasome-dependent degradation of pVHL. (A) 786-O cells stably expressing either WT or deletion mutant (containing amino acids indicated above blot) Flag-tagged pVHL were incubated with cycloheximide (CHX) for 0, 3, or 6 h, or with lactacystin (L) for 6 h, as indicated above blot. Whole cell lysates were normalized for equal protein loading (10 μg) in each lane. Western blotting was performed by using anti-Flag mAb. (B) 786-O cells stably expressing either WT or point mutant (containing amino acids substitutions indicated above blot) Flag-tagged pVHL were analyzed as in A. Position of WT and point mutant Flag-VHLp24(MPR) proteins are indicated by an arrowhead to the left of the blot.