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. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: Biochimie. 2009 Feb 6;91(5):577–585. doi: 10.1016/j.biochi.2009.01.010

Fig. 1.

Fig. 1

H2O2 stimulates phosphorylation of Akt in SH-SY5Y Cells. (A) Serum starved SH-SY5Y cells were treated with 0.1 mM H2O2 for the indicated times. Whole cell lysates were analyzed by Western blotting, using an anti-phospho-Akt antibody (1:1000, upper panel). Blots were then stripped and blotted for Akt protein (1:1000, lower panel). Cells were pre-treated with or without 20 μM LY294002 for 1 h, followed by H2O2 treatment for 4 h (B) or for 24 h (C) and immunoblotted for phospho-Akt as indicated (upper panels). Blots were then stripped and blotted for Akt protein (lower panels). Data are from one of three representative experiments.