Fig. 4.

Bax translocation to the mitochondria following PI3K inhibition. (A–C) Control SH-SY5Y cells; (D–F) cells treated with 0.1 mM H₂O₂ for 24 h; (G–I) cells pre-treated with PI3K inhibitor (20 μM of LY294002, LY) and then treated with 0.1 mM H₂O₂ for 24 h (LY + H₂O₂). Cells were double immunostained with Bax (N-20, red), Cox1 (green) and nuclei were visualized by DAPI (blue). Asterisks indicate cells in inserts. Normal healthy control cells are shown in A and C. In H₂O₂ only treatments (D–F), cells are healthy with intact nuclei and diffuse Bax staining in the cytoplasm. PI3K inhibition resulted in punctate Bax staining (G) and Bax translocation to mitochondria (H) as is evident by yellow signal in the merged image (I). Bars = 10 μm.