Table 2.
Tryptic peptides identified by in-gel trypsin digestion and mass spectrometry
| Band | Collagen chain | Sequence | Mass (M+H) |
|---|---|---|---|
| 1, 2 | α1(II) | VGPP*GANGNP*GPAGPP*GPAGK | 1814.94 |
| GEAGAQGPMGPSGPAGAR | 1568.70 | ||
|
| |||
| 3 | α1(V) | GDP*GPSGPP*GIP*GDDGER | 1725.71 |
| α1(XI) | TGP*P*GPGGVVGPQGPTGETGPIGER | 2304.46 | |
|
| |||
| 4 | α2(XI) | P*GATGQAGPP*GPVGPP*GLP*GLR | 2015.22 |
| GDTGAQGLP*GPP*GEDGER | 1742.74 | ||
|
| |||
| 5 | α1(V) | GPAGAAGPIGIP*GR | 1207.37 |
| GDP*GPSGPP*GIP*GDDGER | 1725.71 | ||
|
| |||
| 6 | α2(XI) | GDTGAQGLP*GPP*GEDGER | 1742.74 |
Identities of bands 1–6 from Figure 3 are shown. Note the presence of peptides from both α1(XI) and α1(V) in band 3 from the heterozygote cartilage.
P*, Hydroxyproline