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. 2009 Apr 9;296(6):G1230–G1237. doi: 10.1152/ajpgi.90508.2008

Fig. 5.

Fig. 5.

Basal levels of p70S6 kinase phosphorylation are increased during chronic intestinal inflammation. A: representative immunoblots of basal p70S6 kinase (Thr389) phosphorylation (pp7056k) in colonic smooth muscle from control and TNBS-treated rats. B: densitometric analysis of basal p70S6 kinase (Thr389) phosphorylation in colonic smooth muscle from control and TNBS-treated rats. Basal p70S6 kinase phosphorylation is significantly increased in smooth muscle cells isolated from the chronically inflamed colons of rats treated with TNBS compared with control (vehicle)-treated rats. The p70S6 kinase (Thr389) phosphorylation was measured by immunoblot analysis in colonic smooth muscle cells isolated from rat colon 7 days following rectal administration of TNBS or vehicle. Results are expressed in relative densitometric units in samples containing equal amounts of total protein. Values represent means ± SE of 4 experiments on separate animals. *P < 0.05 vs. control.