Fig. 4.

Seromuscular stripping method for murine intestine. A: intestinal preparation (duodenum; note pylorus at upper end) is pinned mucosal side down to a cooled Sylgard-cast plate under a dissection stereomicroscope with bottom illumination (magnification = ×7) B: scalpel blade is used to score the seromuscular layer (white dashed line; note occasional full thickness cuts). The seromuscular layer is reflected using fine microforceps by blunt dissection (white arrow). C: higher magnification of reflected seromuscular layer (magnification = ×32).